Point Mutations in the β-Tubulin of Phytophthora sojae Confer Resistance to Ethaboxam.
Identifieur interne : 000415 ( Main/Exploration ); précédent : 000414; suivant : 000416Point Mutations in the β-Tubulin of Phytophthora sojae Confer Resistance to Ethaboxam.
Auteurs : Qin Peng [République populaire de Chine] ; Zhiwen Wang [République populaire de Chine] ; Yuan Fang [République populaire de Chine] ; Weizhen Wang [République populaire de Chine] ; Xingkai Cheng [République populaire de Chine] ; Xili Liu [République populaire de Chine]Source :
- Phytopathology [ 0031-949X ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- effets des médicaments et des substances chimiques : Phytophthora.
- génétique : Phytophthora, Résistance aux substances, Tubuline.
- parasitologie : Maladies des plantes, Soja.
- pharmacologie : Thiazoles, Thiophènes.
- Mutation ponctuelle.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Tubulin.
- chemical , pharmacology : Thiazoles, Thiophenes.
- drug effects : Phytophthora.
- genetics : Drug Resistance, Phytophthora.
- parasitology : Plant Diseases, Soybeans.
- Point Mutation.
Abstract
Ethaboxam is a β-tubulin inhibitor registered for the control of oomycete pathogens. The current study was established to determine the ethaboxam sensitivity of the plant pathogen Phytophthora sojae and investigate the potential for the emergence of fungicide resistance. The effective concentration for 50% inhibition (EC50) of 112 Phytophthora sojae isolates exhibited a unimodal distribution with a mean EC50 for ethaboxam of 0.033 µg/ml. Establishing this baseline sensitivity provided critical data for monitoring changes in ethaboxam-sensitivity in field populations. The potential for fungicide resistance was investigated using adaptation on ethaboxam-amended V8 agar, which resulted in the isolation of 20 resistant mutants. An assessment of the biological characteristics of the mutants including mycelial growth, sporulation, germination rate and pathogenicity indicated that the resistance risk in Phytophthora sojae was low to medium with no cross-resistance between ethaboxam and cymoxanil, metalaxyl, flumorph, and oxathiapiprolin being detected. However, positive cross-resistance was found between ethaboxam and zoxamide for Q8L and I258V but negative cross-resistance for C165Y. Further investigation revealed that the ethaboxam-resistant mutants had point mutations at amino acids Q8L, C165Y, or I258V of their β-tubulin protein sequences. CRISPR/Cas9-mediated transformation experiments confirmed that the Q8L, C165Y, or I258V mutations could confer ethaboxam resistance in Phytophthora sojae and that the C165Y mutation induces high levels of resistance. Taken together, the results of the study provide essential data for monitoring the emergence of resistance and resistance management strategies for ethaboxam, as well as for improving the design of novel β-tubulin inhibitors for future development.
DOI: 10.1094/PHYTO-01-19-0032-R
PubMed: 31559903
Affiliations:
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Ethaboxam.</title><author><name sortKey="Peng, Qin" sort="Peng, Qin" uniqKey="Peng Q" first="Qin" last="Peng">Qin Peng</name><affiliation wicri:level="3"><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Zhiwen" sort="Wang, Zhiwen" uniqKey="Wang Z" first="Zhiwen" last="Wang">Zhiwen Wang</name><affiliation wicri:level="3"><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Fang, Yuan" sort="Fang, Yuan" uniqKey="Fang Y" first="Yuan" last="Fang">Yuan Fang</name><affiliation wicri:level="3"><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Weizhen" sort="Wang, Weizhen" uniqKey="Wang W" first="Weizhen" last="Wang">Weizhen Wang</name><affiliation wicri:level="3"><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, China Agricultural University, 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Pathology, Northwest Agricultural & Forest University, Yangling</wicri:regionArea><wicri:noRegion>Yangling</wicri:noRegion></affiliation></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Drug Resistance (genetics)</term><term>Phytophthora (drug effects)</term><term>Phytophthora (genetics)</term><term>Plant Diseases (parasitology)</term><term>Point Mutation (MeSH)</term><term>Soybeans (parasitology)</term><term>Thiazoles (pharmacology)</term><term>Thiophenes (pharmacology)</term><term>Tubulin (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Maladies des plantes (parasitologie)</term><term>Mutation ponctuelle (MeSH)</term><term>Phytophthora (effets des médicaments et des substances chimiques)</term><term>Phytophthora (génétique)</term><term>Résistance aux substances (génétique)</term><term>Soja (parasitologie)</term><term>Thiazoles (pharmacologie)</term><term>Thiophènes (pharmacologie)</term><term>Tubuline (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Tubulin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Thiazoles</term><term>Thiophenes</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drug Resistance</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora</term><term>Résistance aux substances</term><term>Tubuline</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term><term>Soja</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Thiazoles</term><term>Thiophènes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Point Mutation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Mutation ponctuelle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ethaboxam is a β-tubulin inhibitor registered for the control of oomycete pathogens. The current study was established to determine the ethaboxam sensitivity of the plant pathogen <i>Phytophthora sojae</i> and investigate the potential for the emergence of fungicide resistance. The effective concentration for 50% inhibition (EC<sub>50</sub>) of 112 <i>Phytophthora sojae</i> isolates exhibited a unimodal distribution with a mean EC<sub>50</sub> for ethaboxam of 0.033 µg/ml. Establishing this baseline sensitivity provided critical data for monitoring changes in ethaboxam-sensitivity in field populations. The potential for fungicide resistance was investigated using adaptation on ethaboxam-amended V8 agar, which resulted in the isolation of 20 resistant mutants. An assessment of the biological characteristics of the mutants including mycelial growth, sporulation, germination rate and pathogenicity indicated that the resistance risk in <i>Phytophthora sojae</i> was low to medium with no cross-resistance between ethaboxam and cymoxanil, metalaxyl, flumorph, and oxathiapiprolin being detected. However, positive cross-resistance was found between ethaboxam and zoxamide for Q8L and I258V but negative cross-resistance for C165Y. Further investigation revealed that the ethaboxam-resistant mutants had point mutations at amino acids Q8L, C165Y, or I258V of their β-tubulin protein sequences. CRISPR/Cas9-mediated transformation experiments confirmed that the Q8L, C165Y, or I258V mutations could confer ethaboxam resistance in <i>Phytophthora sojae</i> and that the C165Y mutation induces high levels of resistance. Taken together, the results of the study provide essential data for monitoring the emergence of resistance and resistance management strategies for ethaboxam, as well as for improving the design of novel β-tubulin inhibitors for future development.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31559903</PMID><DateCompleted><Year>2019</Year><Month>12</Month><Day>06</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><Volume>109</Volume><Issue>12</Issue><PubDate><Year>2019</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Point Mutations in the β-Tubulin of <i>Phytophthora sojae</i> Confer Resistance to Ethaboxam.</ArticleTitle><Pagination><MedlinePgn>2096-2106</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-01-19-0032-R</ELocationID><Abstract><AbstractText>Ethaboxam is a β-tubulin inhibitor registered for the control of oomycete pathogens. The current study was established to determine the ethaboxam sensitivity of the plant pathogen <i>Phytophthora sojae</i> and investigate the potential for the emergence of fungicide resistance. The effective concentration for 50% inhibition (EC<sub>50</sub>) of 112 <i>Phytophthora sojae</i> isolates exhibited a unimodal distribution with a mean EC<sub>50</sub> for ethaboxam of 0.033 µg/ml. Establishing this baseline sensitivity provided critical data for monitoring changes in ethaboxam-sensitivity in field populations. The potential for fungicide resistance was investigated using adaptation on ethaboxam-amended V8 agar, which resulted in the isolation of 20 resistant mutants. An assessment of the biological characteristics of the mutants including mycelial growth, sporulation, germination rate and pathogenicity indicated that the resistance risk in <i>Phytophthora sojae</i> was low to medium with no cross-resistance between ethaboxam and cymoxanil, metalaxyl, flumorph, and oxathiapiprolin being detected. However, positive cross-resistance was found between ethaboxam and zoxamide for Q8L and I258V but negative cross-resistance for C165Y. Further investigation revealed that the ethaboxam-resistant mutants had point mutations at amino acids Q8L, C165Y, or I258V of their β-tubulin protein sequences. CRISPR/Cas9-mediated transformation experiments confirmed that the Q8L, C165Y, or I258V mutations could confer ethaboxam resistance in <i>Phytophthora sojae</i> and that the C165Y mutation induces high levels of resistance. Taken together, the results of the study provide essential data for monitoring the emergence of resistance and resistance management strategies for ethaboxam, as well as for improving the design of novel β-tubulin inhibitors for future development.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>Qin</ForeName><Initials>Q</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-7710-4639</Identifier><AffiliationInfo><Affiliation>Department of Plant Pathology, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhiwen</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Yuan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Weizhen</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Xingkai</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xili</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Northwest Agricultural & Forest University, Yangling, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013844">Thiazoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013876">Thiophenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014404">Tubulin</NameOfSubstance></Chemical><Chemical><RegistryNumber>7MP0L17BM8</RegistryNumber><NameOfSubstance UI="C492076">ethaboxam</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004351" MajorTopicYN="Y">Drug Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="Y">Phytophthora</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017354" MajorTopicYN="Y">Point Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013844" MajorTopicYN="Y">Thiazoles</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013876" MajorTopicYN="Y">Thiophenes</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014404" MajorTopicYN="Y">Tubulin</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phytophthora sojae</Keyword><Keyword MajorTopicYN="N">ethaboxam</Keyword><Keyword MajorTopicYN="N">point mutation</Keyword><Keyword MajorTopicYN="N">resistance risk</Keyword><Keyword MajorTopicYN="N">resistant mechanism</Keyword><Keyword MajorTopicYN="N">β-tubulin inhibitor</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>9</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31559903</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-01-19-0032-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Peng, Qin" sort="Peng, Qin" uniqKey="Peng Q" first="Qin" last="Peng">Qin Peng</name></noRegion><name sortKey="Cheng, Xingkai" sort="Cheng, Xingkai" uniqKey="Cheng X" first="Xingkai" last="Cheng">Xingkai Cheng</name><name sortKey="Fang, Yuan" sort="Fang, Yuan" uniqKey="Fang Y" first="Yuan" last="Fang">Yuan Fang</name><name sortKey="Liu, Xili" sort="Liu, Xili" uniqKey="Liu X" first="Xili" last="Liu">Xili Liu</name><name sortKey="Wang, Weizhen" sort="Wang, Weizhen" uniqKey="Wang W" first="Weizhen" last="Wang">Weizhen Wang</name><name sortKey="Wang, Zhiwen" sort="Wang, Zhiwen" uniqKey="Wang Z" first="Zhiwen" last="Wang">Zhiwen Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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